Larvae were dissected and labeled as described previously[26 (link)] using rabbit anti-DGluRIII[26 (link)] (1:1000) and mouse anti-BRP[25 (link)] (1:250) primary antibodies (both from Developmental Studies Hybridoma Bank, Iowa City, IA). We also used mouse anti-Dlg (1:2000) (mAb 4f3), developed by Corey S. Goodman (Renovis, San Francisco, CA, USA) and obtained from the Hybridoma Bank; guinea pig anti-WASP[48 (link)] (1:1000), a gift from Dr. Scherzer (Rehovolt, Israel). Goat Cy5/dylight anti-HRP antibody (1:1000), Cy3- and mouse or rabbit Alexa 488- or Cy3- conjugated secondary antibodies and Alexa 633 or Cy5-conjugated anti-guinea pig antibody (1:1000) were obtained from Jackson ImmunoResearch (West Grove, PA, USA).
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