Glutamine Metabolism Immunity Index for Bladder Cancer

Univariate Cox regression analysis was used to screen out the genes associated with overall survival (OS) of bladder cancer, and multivariate Cox regression analysis was used to establish GMII. The Glutamine Metabolism Immunity Index (GMII) was calculated for each patient according to the following formula: Glutamine Metabolism Immunity Index (GMII) = Coef(Gene1) × Expr(Gene1) + Coef(Gene2) × Expr(Gene2) +…… Coef(Genen) × Expr(Genen). Expr(Genen) represents the expression level of a specific gene, and Coef(Genen) represents the coefficient in multivariate Cox analysis. The prognostic value of the features was verified by KM analysis and Receiver Operation Characteristic (ROC) curve, and the prognostic characteristics were verified by GSE13507. Univariate and multivariate Cox analyses were performed to determine whether the characteristics were independent risk factors. According to the clinical characteristic parameters, the correlation and stratification analysis between GMII and clinical characteristics were performed, and the nomogram was constructed to compare the consistency between predicted and actual survival rates by 1-year, 3-year and 5-year calibration maps.