EGFR Mutation Profiling in Lung Cancer

Genomic DNA was extracted from the tumor tissues of patients with lung adenocarcinoma using the QIAamp Fast DNA Tissue Kit (Qiagen, Valencia, CA, USA) according to the manufacturer’s instructions. Exons 18–21 of the EGFR gene were amplified using the polymerase chain reaction (PCR) and were then subjected to the DNA sequencing reaction using the ABI PRISM 3130XL System (Applied Biosystems, Foster City, CA, USA) as described previously [28 (link)].

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Li J.P., Chang J.T., Ju P.C., Hsieh M.H., Chao Y.H., Tsao T.C., Hsieh M.J, & Yang S.F. (2021). Effect of WW Domain-Containing Oxidoreductase Gene Polymorphism on Clinicopathological Characteristics of Patients with EGFR Mutant Lung Adenocarcinoma in Taiwan. International Journal of Environmental Research and Public Health, 18(24), 13136.

Publication 2021

QIAamp Fast DNA Tissue Kit ABI PRISM 3130XL System

Egfr gene Exons Genomic Lung adenocarcinoma Patients Polymerase chain reaction Prism Tissue Tumor

Corresponding Organization : Changhua Christian Hospital

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Variable analysis

independent variables

Exons 18-21 of the EGFR gene

dependent variables

Genomic DNA extracted from the tumor tissues of patients with lung adenocarcinoma

control variables

The QIAamp Fast DNA Tissue Kit (Qiagen, Valencia, CA, USA)
The polymerase chain reaction (PCR) method
The ABI PRISM 3130XL System (Applied Biosystems, Foster City, CA, USA) for DNA sequencing

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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