MEM was acquired from Hi Media Laboratories, Fetal Bovine Serum (FBS) from Cistron Laboratories, Coimbatore, Tamil Nadu, Inida and Trypsin, Methylthiazolyldiphenyl-tetrazoliumbromide (MTT), and Dimethyl Sulfoxide (DMSO) from Sisco Research Laboratory Chemicals, Mumbai, India. Other chemicals and reagents were obtained from Sigma-Aldrich, Mumbai, India.
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