Newborn Tryptase and Calprotectin Levels

Tryptase was assayed from a blood sample placed into EDTA K2 Vacutainer^® tubes (Becton Dickinson, Franklin Lakes, NJ, USA) during a systematic check-up (at 12 h of life (H12) for premature newborns and on Day 3 (D3) for the Guthrie test for full-term newborns. It was assayed using a fluoroenzymatic technique (immunoCAP method) at the biochemistry laboratory of Clermont-Ferrand University Hospital.
The fecal calprotectin assay was also performed by the biochemistry laboratory on a meconium sample weighing at least 1 g by immunofluorescence using a Liaison XL Dia analyzer (Diasorin, Italy).

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Paysal J., Oris C., Troin U., Limeri P.N., Allard J., Tadrent M., Pereira B., Merlin E., Rochette E., Evrard B., Durif J., Sapin V, & Pons M. (2023). Influence of Perinatal Factors on Blood Tryptase and Fecal Calprotectin Levels in Newborns. Children, 10(2), 345.

Publication 2023

EDTA K2 Vacutainer® tubes

Assay Blood Calprotectin Edta Fecal Immunofluorescence Meconium Newborns Premature Tryptase

Corresponding Organization :

Other organizations : Centre Hospitalier Universitaire de Clermont-Ferrand

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Tryptase level
Fecal calprotectin level

control variables

Time of sample collection (12 h of life for premature newborns and Day 3 for full-term newborns)
Sample type (blood for tryptase, meconium for fecal calprotectin)
Sample collection method (EDTA K2 Vacutainer tubes for blood, at least 1 g meconium sample)
Analytical methods (fluoroenzymatic technique for tryptase, immunofluorescence using Liaison XL Dia analyzer for fecal calprotectin)

positive controls

None mentioned

negative controls

None mentioned

Annotations

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