The 4β-HC, 4β-hydroxycholesterol-d7 (4β-HC-d7, an internal standard), and other oxysterols were purchased from Avanti Polar Lipids (Alabaster, AL, USA). The purity of the standards was >99%. The 4α-HC was obtained from Toronto Research Chemicals (Toronto, ON, Canada). Butylhydroxytoluene (BHT) was obtained from Sigma-Aldrich (St. Louis, MO, USA), and sodium methoxide was obtained from Tokyo Chemical Industry (Tokyo, Japan). All other chemicals were above the analytical grade.
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