RNA Extraction and qPCR Analysis Protocol

The root, stem, leaf, petiole and receptacle samples stored at − 80 °C were used for total RNA extraction with a modified CTAB method [80 ].The cDNA was generated by Primerscript RT reagent Kit with gDNA Erase (Takara) according to the manufacturer’s protocol. Quantitative PCR (qPCR) was performed using SYBR Premix Ex Tag (Takara), with GAPDH as an internal reference gene [81 (link)]. Results were analyzed using the 2^-ΔCT method [81 (link)]. Three biological and three technical replicates were performed and analyzed. Primers used in this study are listed in Additional file 12.

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Wu H., Li H., Chen H., Qi Q., Ding Q., Xue J., Ding J., Jiang X., Hou X, & Li Y. (2019). Identification and expression analysis of strigolactone biosynthetic and signaling genes reveal strigolactones are involved in fruit development of the woodland strawberry (Fragaria vesca). BMC Plant Biology, 19, 73.

Publication 2019

Primerscript RT reagent Kit gDNA Erase SYBR Premix Ex Tag

Biological Cdna Ctab Gapdh Gene Leaf Primers Root Stem

Corresponding Organization : University of Connecticut

Other organizations : Institute of Botany, Beijing Forestry University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Gene expression levels of various samples (root, stem, leaf, petiole, receptacle)

control variables

GAPDH as an internal reference gene for qPCR

controls

No positive or negative controls were explicitly mentioned.

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