Carbapenemase Genes Detection in Acinetobacter

The presence of acquired class D carbapenemases genes common for Acinetobacter spp. (groups OXA-23, OXA-24/40, and OXA-58), as well as class B carbapenemases (metallo-beta-lactamases (MBL) of VIM, IMP, and NDM groups) were determined by real-time PCR using commercial kits “AmpliSensR MDR Acinetobacter-OXA-FL” and “AmpliSensR MDR MBL-FL” (Central Research Institute of Epidemiology, Moscow, Russia). For amplification, a DTPrime 5X1 real-time PCR system (DNA Technology, Moscow, Russia) was used. Strains A. baumannii, A. pittii, and P. aeruginosa, carring the known carbapenemases genes of the listed groupswere used as positive controls. DNA extraction was performed by express method using InstaGeneTM matrix (Bio-Rad, Hercules, CA, USA). Samples of extracted DNA were stored at −20 °C before testing. The results of assessing the sensitivity to antibiotics and determining the genes of various types of carbapenemases have been deposited to the AMRmap website database [49 (link)].