HPLC Analysis of Cornelian Cherry

The chemical analysis of the cornelian cherry extract was performed by high-performance liquid chromatography (HPLC-PDA), as described previously by Kucharska et al. [46 (link)]. The HPLC analysis was performed using a Dionex (Germering, Germany) system equipped with an Ultimate 3000 diode array detector, LPG-3400A quaternary pump, EWPS-3000SI autosampler, TCC-3000SD thermostated column compartment, and controlled by Chromeleon v.7.2 software (Thermo Fisher, Waltham, MA, USA). The HPLC conditions are summarized in Table S1. The compounds were identified by comparing their retention times and UV-Vis spectra with authentic standards of iridoid and phenolic compounds (Extrasynthese, Genay, France) and by comparison with literature data. Iridoids were quantified as loganic acid, anthocyanins as cyanidin 3-O-glucoside, phenolic acids as p-coumaric acid, caffeic acid, and ellagic acid, flavonols as quercetin 3-O-glucoside or kaempferol 3-O-glucoside. The cornelian cherry extract was analyzed in three repetitions.

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Szandruk-Bender M., Nowak B., Merwid-Ląd A., Kucharska A.Z., Krzystek-Korpacka M., Bednarz-Misa I., Wiatrak B., Szeląg A., Piórecki N, & Sozański T. (2023). Cornus mas L. Extract Targets the Specific Molecules of the Th17/Treg Developmental Pathway in TNBS-Induced Experimental Colitis in Rats. Molecules, 28(7), 3034.

Publication 2023

Ultimate 3000 LPG-3400A EWPS-3000SI TCC-3000SD Chromeleon v.7.2 software

Anthocyanins Caffeic acid Cherry Coumaric acids Cyanidin 3 o glucoside Ellagic acid Flavonols Hplc Iridoids Kaempferol 3 o glucoside Loganic acid Quercetin 3 o glucoside Retention

Corresponding Organization : Wroclaw Medical University

Other organizations : Wroclaw University of Environmental and Life Sciences, Rzeszów University

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Variable analysis

independent variables

Cornelian cherry extract

dependent variables

Compounds identified and quantified by HPLC-PDA analysis
Iridoids quantified as loganic acid
Anthocyanins quantified as cyanidin 3-O-glucoside
Phenolic acids quantified as p-coumaric acid, caffeic acid, and ellagic acid
Flavonols quantified as quercetin 3-O-glucoside or kaempferol 3-O-glucoside

control variables

HPLC-PDA conditions as summarized in Table S1
Authentic standards of iridoid and phenolic compounds used for identification and quantification

controls

Positive control: Authentic standards of iridoid and phenolic compounds
Negative control: Not explicitly mentioned

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