Western Blot Analysis of Cell Signaling

Western blot analysis was performed with sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) as previously described [21 (link)]. Antibodies were used against BMAL1 (Abcam, Cambridge, UK, ab3350), E-cadherin (Cell signaling Technology, Inc., Boston, MA, USA, 14472), vimentin (Cell signaling Technology, 73260), β-catenin (Cell signaling Technology, 8480). HSC-70 (Stressgen, CA, USA, SPA-815), β-actin (Cell signaling Technology, 47778) and Lamin β1 (Abcam, ab16048) were used as control for protein loading. Image J 1.4.3 was used to quantification the western results. The t-test was used for statistical analysis.