Quantifying Urinary Albumin and Creatinine

Albumin and creatinine levels in the urine of mice were measured as previously described [19 (link),20 (link)]. Mice were placed in individual metabolic cages for 12 h to collect urine samples after 24 weeks of STZ-induced hyperglycaemia. Albuminuria was determined using a mouse albumin enzyme-linked immunosorbent assay kit (Bethyl Laboratories) according to the manufacturer’s instructions. Urinary creatinine was determined using the Cobasc501 module assay from Roche Diagnostics [14 (link)].

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Rana R., Manoharan J., Gupta A., Gupta D., Elwakiel A., Khawaja H., Fatima S., Zimmermann S., Singh K., Ambreen S., Gadi I., Biemann R., Jiang S., Shahzad K., Kohli S, & Isermann B. (2022). Activated Protein C Ameliorates Tubular Mitochondrial Reactive Oxygen Species and Inflammation in Diabetic Kidney Disease. Nutrients, 14(15), 3138.

Publication 2022

mouse albumin enzyme-linked immunosorbent assay kit

Albumin Assay Creatinine Diagnostics Enzyme linked immunosorbent assay Hyperglycaemia Mouse Urinary

Corresponding Organization : University Hospital Leipzig

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Variable analysis

independent variables

STZ-induced hyperglycaemia

dependent variables

Albuminuria
Urinary creatinine

control variables

Mice were placed in individual metabolic cages for 12 h to collect urine samples

controls

No positive or negative controls were explicitly mentioned in the provided information.

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