pETDUET-1™ DNA (Cat. 71146-3) vector was obtained from Novagen (Merck Biosciences Division, Darmstadt, Germany). The full-length, 1647 bp long, wildtype Human TLK1B gene was obtained through a custom gene synthesis service from GeneScript Biotech Corporation, Nanjing, China. High-Fidelity (HF®) Restriction Endonucleases (BamHI, EcoRI, MfeI and XhoI), CutSmart® Buffer, T4 DNA Ligase and T4 DNA Ligase Reaction Buffer for the cloning procedures were purchased from New England Biolabs (Ipswich, MA, USA). Plasmid DNA Purification Kit and QIAquick® Gel Extraction Kit were purchased from Qiagen (Hilden, Germany). Escherichia coli DH5α™ (Cat. 18265-017) competent cells were purchased from Invitrogen Corporation (Carlsbad, CA, USA) and Rosetta Gami 2™ (DE3) pLysS (Cat. 71352) competent cells were purchased from Novagen (Merck Biosciences Division, Darmstadt, Germany). Ampicillin, Chloramphenicol, Isopropyl-β-D-1-thiogalactopyranoside (IPTG), Luria-Bertani (LB) broth, Luria-Bertani (LB) agar, Trizma® (Tris base), Sodium Chloride (NaCl), Glycerol, Triton-X l00, Tween-20, Imidazole, Tris (2-Carboxyethyl) Phosphine Hydrochloride (TCEP), Acrylamide, N,N′-Methylenebisacrylamide, Tetramethylethylenediamine (TEMED), Magnesium chloride, Ammonium Per Sulphate (APS), Sodium Dodecyl Sulphate (SDS), β-Mercaptoethanol (BME), Bromophenol Blue, Coomassie Brilliant Blue R-250, Non-Fat Milk, Ponceau S, Bovine Serum Albumin (BSA) and Nuclease-Free water were purchased from Sigma-Aldrich (Darmstadt, Germany). Complete™ EDTA-Free Protease Inhibitor tablets (Cat. 04693132001) were obtained from Roche (Basel, Switzerland). Nuvia™ Immobilized Metal Affinity Chromatography (IMAC) Resin charged with Ni2+, Econo-Column® Chromatography Columns and Clarity™ ECL Western Blotting Substrate were purchased from Bio-Rad Laboratories (Hercules, CA, USA). HiLoad 16/600 Superdex 75 pg gel filtration chromatography column was purchased from GE Healthcare (Chicago, Illinois, USA). Amicon Ultra concentrator with a 10 kDa cut-off filter was obtained from Merck Millipore (Darmstadt, Germany). Immun-Blot PVDF Western Blotting Membrane was purchased from Bio-Rad Laboratories (Hercules, CA, USA). His-Tag (D3I10) XP® Rabbit Monoclonal Antibody (Cat. 12698) and Anti-Rabbit IgG HRP-linked Secondary Antibody (Cat. 7074) were obtained from Cell Signaling Technology (MA, USA). ADP-Glo™ Kinase Assay Kit was purchased from Promega Corporation (Madison, WI, USA). Recombinant ASF1a substrate (Cat. PRO-682) for the kinase assay was purchased from ProSpec Technogene Ltd., Ness-Ziona, Israel.
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