Von Frey Mechanical Threshold Assay

Hind paw mechanical threshold was assessed using von Frey filaments (Bioseb, France) as described^{20 (link)}. For facial testing, mice were subjected to a von Frey stimulus applied to the forehead surface, repeated three times (at minimum 30 s interval). The head withdrawal tactile sensory threshold was the lowest force to elicit withdrawal in 2 of 3 trials. Data points were normalized to the control sensory threshold values determined just before minipump implantation (i.e. at day 0) or before SNP injection (i.e. at day 21, H0).

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Bonnet C., Hao J., Osorio N., Donnet A., Penalba V., Ruel J, & Delmas P. (2019). Maladaptive activation of Nav1.9 channels by nitric oxide causes triptan-induced medication overuse headache. Nature Communications, 10, 4253.

Publication 2019

von Frey filaments

Facial Filaments Forehead Head Implantation Mice

Corresponding Organization :

Other organizations : Centre de Recherche en Neurobiologie - Neurophysiologie de Marseille, Neuro-Dol, Centre de Référence Déficits Immunitaires Héréditaires, Hôpital Saint Eloi

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Variable analysis

independent variables

Von Frey filament stimulus applied to the forehead surface

dependent variables

Head withdrawal tactile sensory threshold (the lowest force to elicit withdrawal in 2 of 3 trials)
Hind paw mechanical threshold

control variables

Von Frey filament stimulus was repeated three times with a minimum 30 s interval
Sensory threshold values determined just before minipump implantation (i.e. at day 0) or before SNP injection (i.e. at day 21, H0)

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