Sanger Sequencing of CD36 Gene

We performed direct Sanger sequencing to screen the coding sequence of the CD36 gene in 184 French individuals of European ancestry. The protocol was carried out using the automated ABI Prism 3730xl DNA sequencer in combination with the Big Dye Terminator Cycle Sequencing Ready Reaction kit 3.1 (Applied Biosystems). PCR conditions and primers sequences are available on request. Samples from the FREX project have been sequenced using the Agilent V5 + UTR exome capture kit and genotyped on Illumina Core Exome SNP-chip. Full details of data processing, variant calling, filtering process and variant annotation in ExAC have been previously described^{34 (link)}. GnomAD was quality controlled and analyzed using the Hail open source framework (https://github.com/hail-is/hail). This data set can be accessed via the gnomAD Browser (http://gnomad.broadinstitute.org/).