The procedure used for OCT imaging of mouse retina followed a published protocol.5 (link),27 (link) Briefly, after mice were anesthetized with ketamine (100 mg/kg) and xylazine (6 mg/kg), retina OCT images were captured with Envisu UHR2200 (Bioptigen, Durham, NC, USA). The mouse eye was positioned with the ONH in the center of the OCT scan. Full field volume scans (1.4 mm × 1.4 mm at 1000 A-scan × 100 B-scan × 5) and two radial scans (at horizontal and vertical position and averaged 40 times) were captured. Mice retina were first imaged after ∼20 minutes of adaptation to room light (500 lux) in the procedure room under standard illumination conditions, and again in darkness after overnight dark adaptation. Averaged radial scan images were used for retinal thickness measurement. For each eye, measurements were performed on 4 spots (450 μm from center of ONH at both horizontal and vertical directions) by using the vendor-provided Reader program (Bioptigen), and an averaged number was used as the measurement for the eye. Outer retina length was measured from the outer limiting membrane to the RPE-choroid boundary.