HepG2, HepG2-miR-122 (36 (link)) (obtained from Dr Matthew Evans, Icahn School of Medicine at Mount Sinai), Huh-7.5 and Huh-7.5-ΔmiR-122 (37 (link)) cells (obtained from Dr Charles Rice, Rockefeller University) were maintained in DMEM medium with 10% fetal bovine serum. Sofosbuvir (2′-deoxy-2′-α-fluoro-β-C-methyluridine-5′-monophosphate) was purchased from ChemScene, LLC (Monmouth Junction, NJ). Final dilutions contained 0.1% DMSO. Cell viability was assessed with WST-1 reagent (MilliporeSigma, Burlington, MA) using the manufacturer’s suggested protocol.
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Evaluating Antiviral Activity in Liver Cell Lines
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Corresponding Organization : University of North Carolina at Chapel Hill
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Variable analysis
independent variables
- Cell lines: HepG2, HepG2-miR-122, Huh-7.5, and Huh-7.5-ΔmiR-122
- Cell viability
- DMEM medium with 10% fetal bovine serum
- Final dilutions contained 0.1% DMSO
- No positive or negative controls were explicitly mentioned.
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