Total and glycolytic ATP were detected using a glycolysis/OXPHOS assay kit (G270, Dojindo, Japan) with or without oligomycin (2.5μm) to assess OXPHOS levels as described previously. Mitochondrial ATP was calculated by subtracting glycolytic ATP from total ATP. Luminenscence was measured using a microplate reader Varioscan Lux (Thermo Fisher Scientific) [19 (link)].
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