Micro-CT Morphometric Analysis of Tadpole Liver

A micro-computed tomography (Micro-CT) scan was used to examine the morphological variation of the liver during metamorphic climax. After anesthetization by MS-222, tadpoles were fixed in 4% paraformaldehyde for more than 24 h and stained in I₂ & KI water solutions (respectively, 1% & 2%, w/v) for 12 h [21 (link)]. A Micro-CT scan was conducted on a Quantum GX Micro CT (PerkinElmer, Waltham, MA, USA) with the following parameters: scanning current, 70 eV; 10 μM; field-of-view: 36 × 36 mm for acquisition, 25 × 25 mm for reconstruction; scan duration, 15 min.

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Zhu W., Chang L., Zhao T., Wang B, & Jiang J. (2020). Remarkable metabolic reorganization and altered metabolic requirements in frog metamorphic climax. Frontiers in Zoology, 17, 30.

Publication 2020

Quantum GX Micro CT

Computed tomography Liver Micro ct Mm 36 Ms 222 Paraformaldehyde Reconstruction Scan Tadpoles

Corresponding Organization :

Other organizations : Chengdu Institute of Biology

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Variable analysis

independent variables

Metamorphic climax stage

dependent variables

Morphological variation of the liver

control variables

Anesthetization by MS-222
Fixation in 4% paraformaldehyde for more than 24 h
Staining in I2 & KI water solutions (1% & 2%, w/v) for 12 h
Scanning current: 70 eV; 10 μM
Field-of-view: 36 × 36 mm for acquisition, 25 × 25 mm for reconstruction
Scan duration: 15 min

controls

Positive control: Not mentioned
Negative control: Not mentioned

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