Spinal Cord Injury Transection in Rats

Under ketamine/xylazine anesthesia (80/10 mg/kg, intraperitoneally) and aseptic conditions, animals underwent a dorsal incision and laminectomy at T8 , as previously described^{15 (link),16 (link)}. T9-level transections were performed with micro-scissors. Gel foam was inserted into the lesion site prior to closure of the dorsal muscle with 4–0 suture (Ethicon) and skin with wound clips (Mikrotek, 9 mm autoclip). After surgery, the rats receive daily manual bladder emptying 3/day until voiding reflexively. Pain medication (Meloxicam,1/day for 3 days); and antibiotics (Gentafuse, gentamiacin, SC, 1/day for 3 days; Penject, penicilin G, SC, 1/day for 3 days) were provided per our laboratories standard protocol^{15 (link),16 (link)}.

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Medina-Aguiñaga D., Hoey R.F., Wilkins N.L., Ugiliweneza B., Fell J., Harkema S.J, & Hubscher C.H. (2023). Mid-lumbar (L3) epidural stimulation effects on bladder and external urethral sphincter in non-injured and chronically transected urethane-anesthetized rats. Scientific Reports, 13, 12258.

Publication 2023

4–0 suture

Anesthesia Animals Antibiotics Aseptic Bladder Clips Dorsal muscle Ketamine Laminectomy Medication Meloxicam Pain Rats Skin Surgery Suture Wound Xylazine

Corresponding Organization : University of Louisville

Other organizations : Case Western Reserve University, University School, MetroHealth, Neurological Surgery

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Variable analysis

independent variables

Transection of the spinal cord at T9 level

dependent variables

Voiding reflex recovery

control variables

Anesthesia (ketamine/xylazine, 80/10 mg/kg, intraperitoneal)
Aseptic conditions
Dorsal incision and laminectomy at T8
Insertion of gel foam into the lesion site
Closure of the dorsal muscle and skin
Daily manual bladder emptying
Pain medication (Meloxicam, 1/day for 3 days)
Antibiotics (Gentafuse, gentamicin, SC, 1/day for 3 days; Penject, penicillin G, SC, 1/day for 3 days)

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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