We used a standard scoring system (murine
sepsis score) to assess the symptoms after LPS challenge (see Table S4).33 (link) PET/CT
imaging scans were carried out on GNEXT PET/CT (Sofie Biosciences)
or Inveon D-PET (dedicated PET, Siemens) scanners. After a bolus intravenous
injection of 100–500 μCi (3.7–18.5 MBq) of 11C-MGX-10S into control and LPS
mice (6.24–31.21 pmol), a 60 min dynamic PET scan was performed
after administration of 11C-MGX-10S. The acquired list mode data were reconstructed into 19 frames (4
× 15, 4 × 60, and 11 × 300 s) using Iterative reconstruction
method 3DOSEM-MAP (3D ordered-subset expectation maximization followed
by fast maximum a posteriori (fastMAP) MAP (2; MAP subsets, 16; MAP
iterations, 18). The TAC normalized to the injected dose was computed
and expressed as %ID/g (% injected dose per gram). Image analysis
was performed using commercial software (Scintica VivoQuant version
4.0), which is used to visualize radiotracer uptake and define the
volumes of interest. MIP PET/CT images were created using another
vendor software, Inveon Research Workplace software (Siemens).