U343, U87, U87ΔEGFR, LN229, Gli36 EGFR-H2B-RFP, X12 human glioma cells and Vero cells were maintained as described (20 (link)–22 (link)). Mouse monoclonal anti-chondroitin-4-sulfate antibody (clone BE-123, MP Biomedicals Inc, Aurora, OH) was used to probe for Chase functionality. Tumor bearing sections were labeled with Wisteria floribunda lectin (WFL, Vector Labs Inc., Burlingame, CA), anti vimentin (clone SP20, Lab Vision, Fremont, CA). Oncolytic viruses rHsvQ, rHsvQLuc and rQNestin34.5 have been previously described (23 (link)–24 (link)). Genomic DNA from Proteus vulgaris (ATCC number 9920D) was used as template to clone Chase-ABC-I cDNA as described (25 (link)). The PCR product was sub-cloned into pSecTag/FRT/V5 His-Topo vector (Invitrogen, Carlsbad, CA) and used to generate OV-Chase as previously described (24 (link)). Cytotoxicity of viruses was assessed by a standard crystal violet assay (26 (link)).
Protocol detail
Chondroitinase-Expressing Oncolytic Viruses
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Anti antibody
Assay
Cdna
Cells
Chondroitin 4 sulfate
Crystal violet
Cytotoxicity
Egfr
Genomic
Glioma
Human
Mouse
Oncolytic viruses
Proteus vulgaris
Topo
Tumor
Vector
Vero cells
Vimentin
Viruses
Vision
Wisteria floribunda lectin
Top 5 similar protocols
Protocol cited in 5 other protocols
Variable analysis
independent variables
- Oncolytic viruses rHsvQ, rHsvQLuc and rQNestin34.5
- OV-Chase
- Cytotoxicity of viruses
- U343, U87, U87ΔEGFR, LN229, Gli36 EGFR-H2B-RFP, X12 human glioma cells and Vero cells
- Mouse monoclonal anti-chondroitin-4-sulfate antibody (clone BE-123, MP Biomedicals Inc, Aurora, OH)
- Tumor bearing sections labeled with Wisteria floribunda lectin (WFL, Vector Labs Inc., Burlingame, CA), anti vimentin (clone SP20, Lab Vision, Fremont, CA)
- Genomic DNA from Proteus vulgaris (ATCC number 9920D)
- None specified
- None specified
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!