The leaves of the following plants were used for leaf clearing: C. obtusa, C. morifolium, C. sativus, C. fortunei, N. benthamiana, N. tabacum, P. x hybrida, P. frutescens and S. lycopersicum leaves were kindly provided by Dr. M. Notaguchi (Nagoya University). Oryza sativa leaf was kindly provided by Dr. R.D. Kasahara (Fujian Agriculture and Forestry University). We used A. thaliana accession Columbia and T. fournieri cv. ‘Blue and White’. The following transgenic lines were also used: UBQ10p::H2B–mClover of A. thaliana (Kurihara et al. 2015 (link)) and UBQ10p::sGFP, UBQ10p::H2B–mClover and LAT52pro::mTFP1 of N. benthamiana (Nagahara et al. 2021 ).
Arabidopsis thaliana seeds were sown on plates containing half-strength Murashige and Skoog salts (Duchefa Biochemie B.V., Haarlem, The Netherlands), 0.05% MES-KOH (pH 5.8), 1× Gamborg’s vitamin solution (Sigma, St. Louis, MO, USA) and 1% agar. The plates were incubated in a growth chamber at 22°C under continuous lighting after cold treatment at 4°C for 2–3 d. Two-week-old seedlings were transferred to the soil (Sakata no Tane; Sakata Seed, Yokohama, Japan) and grown at 21–25°C under long-day conditions (16-h light/8-h dark).
Arabidopsis thaliana seeds were sown on plates containing half-strength Murashige and Skoog salts (Duchefa Biochemie B.V., Haarlem, The Netherlands), 0.05% MES-KOH (pH 5.8), 1× Gamborg’s vitamin solution (Sigma, St. Louis, MO, USA) and 1% agar. The plates were incubated in a growth chamber at 22°C under continuous lighting after cold treatment at 4°C for 2–3 d. Two-week-old seedlings were transferred to the soil (Sakata no Tane; Sakata Seed, Yokohama, Japan) and grown at 21–25°C under long-day conditions (16-h light/8-h dark).
