Tibial Growth Plate Histomorphometry

Stained transverse sections of tibiae were viewed using a light microscopy eclipse E400 Nikon with ×10, ×20, or ×40 objectives, using light filters. Images were captured by a high-resolution camera (Olympus DP 71) controlled by Cell A software (Olympus). Longitudinal median sections from the proximal tibia growth plate were subjected to histological staining and measurements. The thickness of the total GPs, proliferative zone (PZ) and hypertrophic zone (HA) as well as the number of cells were measured using a Cell A software (Olympus) with a measuring tool feature. Measurements were performed on these sections from 5 different animals from each group. In each slide, 10 random locations throughout the GPs were selected and measured [18 (link),19 (link)].

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Rozner R., Vernikov J., Griess-Fishheimer S., Travinsky T., Penn S., Schwartz B., Mesilati-Stahy R., Argov-Argaman N., Shahar R, & Monsonego-Ornan E. (2020). The Role of Omega-3 Polyunsaturated Fatty Acids from Different Sources in Bone Development. Nutrients, 12(11), 3494.

Publication 2020

Cell A software

Animals Cell Growth plate Hypertrophic Light Light microscopy Tibia

Corresponding Organization : Hebrew University of Jerusalem

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Variable analysis

independent variables

Viewing techniques (light microscopy eclipse E400 Nikon with ×10, ×20, or ×40 objectives, using light filters)

dependent variables

Thickness of the total growth plates (GPs)
Thickness of the proliferative zone (PZ)
Thickness of the hypertrophic zone (HA)
Number of cells in the GPs

control variables

Longitudinal median sections from the proximal tibia growth plate
Histological staining of the sections
Measurements performed on sections from 5 different animals from each group
10 random locations throughout the GPs selected and measured in each slide

controls

No positive or negative controls were explicitly mentioned in the input protocol.

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