HPLC Analysis of Polycyclic Aromatic Hydrocarbons

The HPLC analysis was performed on a SHIMADZU LC-20AT series HPLC system (Kyoto, Japan) with RF-20A fluorescent spectrophotometric detector and SPD-M20A photodiode array ultraviolet-visible light detector. A Zorbax Eclipse PAH (4.6 × 150 mm i.d., 5 μm, Agilent Technologies) column was used for PAHs separation. The column temperature was 30 °C. The mobile phase consisting of ACN and water was in gradient mode (Table 1). And the initial flow rate was 2 mL min⁻¹. The injection volume was 20 μL. ANY was detected by DAD at 228 nm, and the other PAHs were detected by FLD. The excitation and emission wavelengths of FLD were summarized in Table 1.