After exposure to redox metals for 24 h, iFLAG-α-Syn SHSY5Y cells, SNCA-tri iPSCs, and respective controls were harvested for genomic DNA isolation using DNeasy blood and tissue kit (Qiagen #69504) as per the manufacturer’s instructions. Genome integrity was assessed using LongAmp Taq DNA polymerase (NEB) to amplify a 10.4 kB region of the HPTR gene with the following primers: 5’-TGG GAT TAC ACG TGT GAA CCA ACC-3’ and 5’-GCT CTA CCC TCT CCT CTA CCG TCC-3’ [39 ]. PCR was performed at 94°C for 3 min followed by 25cycles at 94°C for 30 s, 58°C for 40 s, and 65°C for 10 min, and a final elongation step for 15 min. A small region (250 bp) of the HPRT gene was amplified using Jump-Start RedTaq DNA polymerase (Sigma) to normalize amplification obtained from the large fragment using the following primers: 5’-TGC TCG AGATGT GAT GAA GG-3’ and 5’-CTG CAT TGT TTT GCC AGT GT-3’ [40 (link)]. All amplified products were separated by electrophoresis in 0.8% agarose gel and stained with ethidium bromide. Relative DNA amplification percentage was calculated by gel band density analysis using ImageJ software or Pico Green dsDNA assay (Invitrogen, p7589).