Protocol detail

Genetic Tools for Drosophila Gustatory Receptors

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Flies were raised on standard cornmeal fly food at 25°C and 70% relative humidity. The following fly lines were used: Gr5a-GAL4, Gr43a-GAL4, Gr64a-GAL4, Gr61a-GAL4, Gr64c-GAL4, Gr64d-GAL4, and Gr64e-GAL4 (ref^{16 (link)}); Gr64f^LexA and Gr43a^GAL4 (ref^{13 (link)}); UAS-GCaMP3 (ref⁴³); UAS-KIR2.1 (ref^{37 (link)}); UAS-TdTomato and UAS-GFP (Bloomington stock center); poxn^Δ^M22-B5 (ref^{44 (link)}); poxn⁷⁰ (ref ^{28 (link)}).

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LeDue E.E., Chen Y.C., Jung A.Y., Dahanukar A, & Gordon M.D. (2015). Pharyngeal sense organs drive robust sugar consumption in Drosophila: Pharyngeal taste in adult Drosophila. Nature communications, 6, 6667.

Publication 2015

UAS-GCaMP3 UAS-KIR2.1 UAS-TdTomato and UAS-GFP

Food Humidity Tdtomato

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Variable analysis

independent variables

Gr5a-GAL4
Gr43a-GAL4
Gr64a-GAL4
Gr61a-GAL4
Gr64c-GAL4
Gr64d-GAL4
Gr64e-GAL4
Gr64f^LexA
Gr43a^GAL4
UAS-GCaMP3
UAS-KIR2.1
UAS-TdTomato
UAS-GFP
Poxn^ΔM22-B5
Poxn^70

dependent variables

Not explicitly mentioned

control variables

Flies were raised on standard cornmeal fly food at 25°C and 70% relative humidity

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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