Spheroids were generated as previously reported [25 (link)]. Briefly, cells resuspended in endothelial basal medium (EBM) containing 0.24% high viscosity methylcellulose (Sigma-Aldrich), and were seeded in 96-well round bottom non-adherent plates. Cells were incubated overnight at 37 °C. Each spheroid contained 1 × 103 cells. Individual spheroids were harvested, embedded in rat tail collagen type 1 gel (Corning, Seneffe, Belgium), supplemented with EBM, and incubated for 48 h at 37 °C. To quantify germination, the average number of shoots under each condition was calculated.
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