Quantifying Candida albicans Morphogenesis

C. albicans SC5314 were grown overnight in YPD medium. And then, 1 × 10⁶ cells/mL with or without compounds were incubated in RPMI 1640 medium, GlcNAc (0.5% GlcNAc, 0.5% peptone, 0.3% KH₂PO₄) or 10% FBS YPD medium at 37°C for 4 h. Inhibition quantification of the yeast-to-hyphal-form transition was accomplished by counting the number of individual budded cells versus the number of hyphae in the population as previously described [26 (link)]. More than 100 cells were counted for each well in duplicate, and all assays were repeated for five times. Images of cells were obtained using a microscope. The experiments were performed in triplicate.