The human malignant melanoma cell line WM451 was purchased from the Cell Bank of Central South University. The cells were incubated in DMEM (Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% fetal bovine serum (Gibco; Thermo Fisher Scientific, Inc.) at 37°C in a humidified incubator with 5% CO2. Subsequently, different concentrations of tepotinib (1, 2, 5, 10, 20 and 50 ng/ml; Selleck Chemicals) were used to treat WM451 cells for 48 h (13 (link)). For tepotinib + hepatocyte growth factor (HGF; R&D Systems, Inc.)-treated group, 50 ng/ml of HGF (a natural agonist of MET) and 10 ng/ml tepotinib were concurrently administered to WM451 cells for 48 h.
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Tepotinib Inhibits Melanoma Cell Growth
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Other organizations : Chifeng University
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Variable analysis
independent variables
- Tepotinib concentrations (1, 2, 5, 10, 20 and 50 ng/ml)
- Tepotinib (10 ng/ml) + Hepatocyte growth factor (HGF; 50 ng/ml)
- Cell viability/proliferation of WM451 cells
- WM451 cell line
- DMEM culture medium supplemented with 10% fetal bovine serum
- Incubation at 37°C in a humidified incubator with 5% CO2
- Treatment duration of 48 hours
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