Geniposide and Lactobacillus casei Synergistic Effects

HOK or HSC-3 cells were cultured in the dish which contained sterilized DMEM medium, and the concentration of cells was adjusted to 2 × 10⁴/dish. Then the cells solution was added into the 96-well plate with 50 μL per well, the cells were cultured with 5% CO₂ at 37 °C for 24 h. Sterile geniposide and LcS were added into the DMEM medium to prepare geniposide-L (25 μg/mL), geniposide-H (50 μg/mL), geniposide-L + LcS (1.0 × 10⁶ CFU/mL) and geniposide-H + LcS solutions, then these solutions were added into the 96-well plate with 50 μL per well. In the meantime, 50 μL of culture solution was added to the control group and cultured in a CO₂ incubator for 48 h. Subsequently, the untreated cells (control group) was added to MTT solution after the removal of clear solution and thereafter incubated for 4 h. In the blank control group, 100 μL of DMSO was added after the removal of the supernatant, followed by shocking for 30 min. The microplate reader was used for detection at 570 nm (680 Microplate Reader, Bio-Rad, Hercules, CA, USA) [13 (link)].