The UCSD Institutional Animal Care and Use Committee (Protocol #S00227M) approved all animal protocols and procedures. Wild-type (WT) C57BL/6 mice aged 8–10 weeks were purchased from Jackson Labs. Epithelial tissue-specific and myeloid-specific knockouts of HIF-1α was accomplished by back-crossing C57Bl/6 HIF-1αfl/fl mice (Jackson Laboratory, Stock No. 007561) with K14cre+ (epithelial cell specific, Jackson Laboratory, Stock No. 004782) or LysMcre+ transgenic mice (myeloid lineage, Jackson Laboratory, Stock No. 004781). Cre-negative HIF-1αfl/fl littermates were used as controls for all experiments [25 (link), 32 (link), 39 (link)]. To ensure statistical power in compliance with ethical guidelines, at least 5 mice per group were used. Treatment groups were assigned randomly and housed in separate cages. Mice were allowed to eat and drink ad libitum.
Protocol detail
Genetic Knockout Mouse Model for HIF-1α
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Animal
C57bl mice
Epithelial cell
Institutional animal care and use committee
Mice
Tissue specific
Transgenic mice
Corresponding Organization :
Other organizations : University of California, San Diego, Baylor College of Medicine, University of California, Santa Cruz
Top 5 similar protocols
Variable analysis
independent variables
- Epithelial tissue-specific knockout of HIF-1α
- Myeloid-specific knockout of HIF-1α
- Not explicitly mentioned
- Age of mice (8-10 weeks)
- Mouse strain (C57BL/6)
- Cre-negative HIF-1α^fl/fl littermates
- Positive control: Cre-negative HIF-1α^fl/fl littermates
- Negative control: Not explicitly mentioned
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!