WT or BM8 mice were irradiated with 900 Rads and rested for 4 hours before reconstitution with either WT, BM8, or CD11c-DTR bone marrow. Bone marrow was isolated and red blood cells were lysed prior to intravenous transfer. At 12 weeks post reconstitution, mice were immunized as described above. For CD11c DTR experiments, mice were vaccinated and 100ng of diptheria toxin (DT) (Sigma Aldrich) was injected intraperitoneally at the indicated time points as previously described 23 (link).
Protocol detail
Investigating CD11c+ Cell Function in Immunization
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Other organizations : University of Colorado Denver
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Variable analysis
independent variables
- Mouse strain (WT or BM8)
- Bone marrow reconstitution (WT, BM8, or CD11c-DTR)
- Time of diptheria toxin (DT) injection (for CD11c DTR experiments)
- Immune response after vaccination (not explicitly mentioned)
- Radiation dose (900 Rads)
- Resting period after irradiation (4 hours)
- Bone marrow isolation and red blood cell lysis prior to transfer
- Time point of evaluation (12 weeks post reconstitution)
- Positive control: WT mice
- Negative control: Not explicitly mentioned
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