Lipidomics Analysis of Brain Tissue

Quantitative analysis was performed on a triple-quadrupole mass spectrometer (Thermo Fisher Scientific) equipped with an automated nanospray apparatus NanoMate and Xcalibur system^{61 (link)–63 (link)}. Internal standards for quantification of individual molecular species of the major lipid classes are added to each brain tissue sample at the start of extraction procedure. Liquid Chromatography/Electro Spray Ionization Lipidomics–Mass Spectrometry (LC/ESI-MS) Lipidomics was performed on a Dionex HPLC system/UltiMate 3000 mobile phase pump, equipped with degassing unit and autosampler^{43 (link), 64 (link), 65 (link)}.

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Nam K.N., Mounier A., Wolfe C.M., Fitz N.F., Carter A.Y., Castranio E.L., Kamboh H.I., Reeves V.L., Wang J., Han X., Schug J., Lefterov I, & Koldamova R. (2017). Effect of high fat diet on phenotype, brain transcriptome and lipidome in Alzheimer’s model mice. Scientific Reports, 7, 4307.

Publication 2017

triple-quadrupole mass spectrometer NanoMate Xcalibur system

Brain Hplc Lipid Liquid chromatography Mass spectrometry Tissue

Corresponding Organization :

Other organizations : University of Pittsburgh, Discovery Institute, Sanford Burnham Prebys Medical Discovery Institute, University of Pennsylvania

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Variable analysis

independent variables

Type of mass spectrometer (Thermo Fisher Scientific triple-quadrupole)
Use of automated nanospray apparatus NanoMate
Use of Xcalibur system

dependent variables

Quantitative analysis of individual molecular species of the major lipid classes

control variables

Addition of internal standards for quantification of individual molecular species of the major lipid classes at the start of the extraction procedure
Use of Liquid Chromatography/Electro Spray Ionization Lipidomics–Mass Spectrometry (LC/ESI-MS) Lipidomics with a Dionex HPLC system/UltiMate 3000 mobile phase pump, equipped with degassing unit and autosampler

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