Western blot analysis was carried out as previously described [63 (link)]. Primary antibodies used in these studies were: cleaved PARP, cleaved caspase-3, phospho-histone H2A.X (Ser139), PLK1, PSMB5 and c-Myc (Cell Signaling Technology, Danvers, MA), Mcl-1 (BD Biosciences, San Jose, CA), α-tubulin (EMD Millipore, Billerica, MA), actin (Sigma-Aldrich, St. Louis, MO), NOXA (Enzo Life Sciences, Farmingdale, NY).
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