After treatment, LAPC4-KD cells were washed and fixed with 1% paraformaldehyde (Sigma-Aldrich) followed by blocking with 1% BSA for 1 h. Cells were probed with LC3B antibody (Cell Signaling) at room temperature for 1 h and then incubated with Alexa Fluor 555-conjugated anti-rabbit antibody (Invitrogen) for 1 h (Xu et al., 2007 (link)). Nuclei were counterstained with Hoechst 33342 for 10 min. The stained cells were then analyzed using a fluorescence microscope (Carl Zeiss) as described previously (Liao et al., 2017 (link)).
Lin H.J., Liu H.H., Lin C.D., Kao M.C., Chen Y.A., Chiang-Ni C., Jiang Z.P., Huang M.Z., Lin C.J., Lo U.G., Lin L.C., Lai C.K., Lin H., Hsieh J.T., Chiu C.H, & Lai C.H. (2017). Cytolethal Distending Toxin Enhances Radiosensitivity in Prostate Cancer Cells by Regulating Autophagy. Frontiers in Cellular and Infection Microbiology, 7, 223.
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to
get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
