Colonic Cytokine and MPO Quantification

The colonic tissue lysate was blended and homogenised as described by [23 (link)]. The expression of pro-inflammatory cytokines INF-γ, IL-6 and TNF-α, were quantified using V-Plex Assay Plates (Meso Scale Diagnostics; Rockville, MD, USA) and analyse as per the manufacturer’s. MPO activity was detected using o-phenylenediamine dihydrochloride as substrate and the data were interpolated from an MPO standard curve (Sigma). Expression of cytokines and MPO was represented as per milligram or U per milligram, respectively, relative to colonic protein.

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Tambuwala M.M., Khan M.N., Thompson P, & McCarron P.A. (2018). Albumin nano-encapsulation of caffeic acid phenethyl ester and piceatannol potentiated its ability to modulate HIF and NF-kB pathways and improves therapeutic outcome in experimental colitis. Drug Delivery and Translational Research, 9(1), 14-24.

Publication 2018

V-Plex Assay Plates MPO standard curve

Assay Colonic Cytokines Diagnostics Inflammatory O phenylenediamine Protein Tissue Tnf α

Corresponding Organization : University of Ulster

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Expression of pro-inflammatory cytokines INF-γ, IL-6 and TNF-α
MPO activity

control variables

None explicitly mentioned

controls

MPO standard curve (Sigma)

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