After four weeks of Lir administration, the blood pressure (BP) of conscious animals was recorded using the tail-cuff plethysmography (BP-98A, Softron Corporation, Beijing, China). The measurements were recorded in preheated chambers at 36–37°C for 10–15 min following the training sessions.
Using the Vevo 2100 system with an MS400 linear array transducer (VisualSonics, ON, Canada), the procedure of transthoracic echocardiography was performed on all mice. Briefly, mice were anesthetized using 2% isoflurane and kept warm using a heating pad (37°C). A depilatory cream was used to remove the chest hair, and a layer of acoustic coupling gel was applied to the thoracic region. RVFWT was measured from the parasternal short-axis view at the midpapillary level of the left ventricle. The pulmonary blood outflow was recorded by pulse-wave Doppler echocardiogram at the level of the aortic valve in the short axis view to measure the values of PAT, PET, and mPAP. Next, the LAD, the left ventricular ejection fraction (LVEF), and LVEDD were measured as described previously [20 (link)].
Using the Vevo 2100 system with an MS400 linear array transducer (VisualSonics, ON, Canada), the procedure of transthoracic echocardiography was performed on all mice. Briefly, mice were anesthetized using 2% isoflurane and kept warm using a heating pad (37°C). A depilatory cream was used to remove the chest hair, and a layer of acoustic coupling gel was applied to the thoracic region. RVFWT was measured from the parasternal short-axis view at the midpapillary level of the left ventricle. The pulmonary blood outflow was recorded by pulse-wave Doppler echocardiogram at the level of the aortic valve in the short axis view to measure the values of PAT, PET, and mPAP. Next, the LAD, the left ventricular ejection fraction (LVEF), and LVEDD were measured as described previously [20 (link)].
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