In vitro propagation and transformation of potato and tomato

The tetraploid potato cultivar Desiree (ZPC, Joure, The Netherlands) was in vitro propagated in a controlled environmental chamber at 19 °C under a 16 h light/8 h dark photoperiod and transformed as previously described [25 (link)]. Plant regeneration was performed using a selection medium containing 250 µg mL⁻¹ cefotaxime (Duchefa, Haarlem, The Netherlands), 100 µg mL⁻¹ timentin® (Duchefa, Haarlem, The Netherlands) and 50 µg mL⁻¹ kanamycin (Duchefa, Haarlem, The Netherlands).
Plants from the WVA106 tomato cultivar were cultured in sterile conditions in a growth chamber with controlled temperatures of 22 °C/18 °C under a 16 h/8 h (day/night) photoperiod. Agrobacterium-mediated transformation using the C58 pGV2260 strain was performed on cotyledon segments from 8-12 day-old seedlings, as previously described [29 (link)]. Plant regeneration was performed using a selection medium containing 225 µg mL⁻¹ timentin® (Duchefa, Haarlem, The Netherlands) and 100 µg mL⁻¹ kanamycin (Duchefa, Haarlem, The Netherlands).