Purification of SOSIP.664-His and gp41ECTO-His Proteins
Corresponding Organization : Scripps Research Institute
Protocol cited in 24 other protocols
Variable analysis
- Supernatants from cells transiently expressing SOSIP.664-His gp140 or gp41ECTO-His proteins
- Binding of the expressed proteins to Ni2+-nitrilotriacetic acid (Ni-NTA) coated Hissorb 96-well plates
- Dilution of supernatants in TBS/10% FCS
- Incubation time of 2 hours with Ni-NTA coated plates
- The subsequent procedures were exactly as described above for the D7324-capture ELISA
- Not explicitly mentioned
- Not explicitly mentioned
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