Supernatants from cells transiently expressing SOSIP.664-His gp140 or gp41ECTO-His proteins were diluted 1∶2 in TBS/10% FCS and incubated for 2 h with Ni2+-nitrilotriacetic acid (Ni-NTA) coated Hissorb 96-well plates (Qiagen, Venlo, The Netherlands) [32] (link), [33] (link), [72] (link), [73] (link). The subsequent procedures were exactly as described above for the D7324-capture ELISA.
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