The murine thymoma cell line EL4 was cultured in complete DMEM (Hiltensperger et al., 2021 (link)). EL4 cells were costimulated under Th17-skewing conditions for 72 h. Cells were then exposed to PBS or IL-1β (25 ng/ml; Miltenyi) in the presence of actinomycin D (2 µg/ml; Tocris Bioscience) for another 16 h of culture.
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