Retinal Vein Occlusion and Penl-XBIR3 Evaluation

Example 3

Penl-XBIR3 eyedrops were delivered immediately after RVO and at 24 h. At 48 h, the eyes were imaged via OCT.

FIG. 4 presents images from individual animals (2 control, 2 RVO, 4 RVO+Penl-XBIR3). For each animal there are three sets of OCT and brightfield images. The brightfield image has a horizontal line showing the level of the OCT.

Four hours after RVO, mouse retinas were harvested for western blot to detect activated caspase-9 (c1Casp9) (FIG. 5, left panel). The blot showed a 10-fold induction of c1Casp9.

To show target engagement, after RVO, mice were given Penl-XBIR3 and retinas were harvested and immunoprecipitated with anti-His followed by western blot for clCasp9 (FIG. 5, right panel). There was a 21-fold increase in binding of XBIR3 and clCasp9 by 4 h and a 45-fold increase by 24 h.