Example 3
Penicillium roqueforti spores were suspended in water. Four DEE chemical compositions were evaluated: (1) 0.06 M copper (II) ions in water, (2) 1 wt.-% surfactant and 10 wt.-% PCSR, (3) 1 wt.-% surfactant and 1 wt.-% PCSR, and (4) 0.5 wt.-% bleach. OxiClean was used as the PCSR and Tween 80 as the surfactant. Clorox was used as bleach. Each DEE composition was added to 0.1 mg/ml suspension of mold spores and exposed to 2.45 GHz microwave for 10 s. After exposure, the cells were centrifuged, washed to remove the DEE chemicals and then plated on Petrifilm and cultured. With the DEE composition 0.06 M copper (II) ions in water and 1 wt.-% surfactant and 10 wt.-% sodium percarbonate, a 6-7 log reduction in P. roqueforti spores (6-7 log kill levels) was realized.
