Protocol detail

Protein Extraction and Characterization

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Maltodextrin, bovine hemoglobin, L-leucine, L-tyrosine, pepsin from porcine gastric mucosa, bovine serum albumin (BSA), Folin–Ciocalteu phenol reagent, and 2,4,6-trinitrobenzenesulfonic (TNBS) acid were purchased from Sigma-Aldrich (St Louis, MO, USA). Acetone, HCl, sodium sulfite, and sodium hydroxide were purchased from LabScan (RCI Labscan Ltd., Bangkok, Thailand). Trichloroacetic acid (TCA), potassium sulfate (K₂SO₄), sodium chloride (NaCl), magnesium chloride (MgCl₂), and lithium chloride (LiCl) were procured from Loba Chemi (Mumbai, Maharashtra, India). Electrophoresis chemicals were acquired from BioRad (Richmond, VA, USA). Whey protein isolate was purchased from ProflexTM (Power Corp. Co., Ltd., Bangkok, Thailand). Threadfin bream (24–48 h after capture) were bought from a local market. Kidney bean protein isolate (KBPI) was prepared as described by Gulzar et al. [15 (link)] with the aid of the pH shift process.

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Patil U., Nikoo M., Zhang B, & Benjakul S. (2022). Freeze-Dried Tuna Pepsin Powder Stabilized by Some Cryoprotectants: In Vitro Simulated Gastric Digestion toward Different Proteins and Its Storage Stability. Foods, 11(15), 2292.

Publication 2022

Maltodextrin bovine hemoglobin L-leucine L-tyrosine pepsin bovine serum albumin Folin–Ciocalteu phenol reagent Acetone sodium hydroxide

Acetone Bovine Bovine serum albumin Electrophoresis Folin Gastric mucosa Hemoglobin Kidney bean L leucine L tyrosine Lithium chloride Magnesium chloride Maltodextrin Nacl Pepsin Phenol Porcine Potassium sulfate Protein Sodium hydroxide Sodium sulfite Trichloroacetic acid Trinitrobenzenesulfonic acid Whey protein

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Variable analysis

independent variables

Maltodextrin
L-leucine
L-tyrosine
Kidney bean protein isolate (KBPI)

dependent variables

Protein content
Protein characterization

control variables

Bovine hemoglobin
Pepsin from porcine gastric mucosa
Bovine serum albumin (BSA)
Folin–Ciocalteu phenol reagent
2,4,6-trinitrobenzenesulfonic (TNBS) acid
Acetone
Sodium sulfite
Sodium hydroxide
Trichloroacetic acid (TCA)
Potassium sulfate (K2SO4)
Sodium chloride (NaCl)
Magnesium chloride (MgCl2)
Lithium chloride (LiCl)
Electrophoresis chemicals
Whey protein isolate
Threadfin bream (24–48 h after capture)

positive controls

Bovine hemoglobin
Bovine serum albumin (BSA)

negative controls

Whey protein isolate

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