Control and 11778(G>A) LHON mutation carrying osteosarcoma cybrid cells were gifts of Valerio Carelli and Andrea Martinuzzi.16 (link) Cells were cultured in Dulbecco's modified Eagle's medium (DMEM; Corning, Inc., Corning, NY, USA) supplemented with 2 mM l -glutamine, 100 mM sodium pyruvate, 10% fetal bovine serum (Corning, Inc.), 50 μg/mL uridine and antibiotics (50 units/mL of penicillin/50 μg/mL of streptomycin; Gibco, Invitrogen, Carlsbad, CA, USA) under humidified 5% CO2 at 37°C.
The human TM–derived cell line, HTM3, was a gift from Alcon Laboratories.2 (link),27 (link) Trabecular cells were maintained in serum-free DMEM (Gibco) supplemented with 4 mMl -glutamine, 10% fetal bovine serum, and 50 μg/mL gentamicin, under humidified 5% CO2 atmosphere at 37°C.
Human corneal epithelial progenitor cells were obtained from Zen-Bio, Inc. (Cat# HCEP; Research Triangle Park, NC, USA). These cells were cultured in epithelial culture medium (CnT-Prime; Zen-Bio, Inc.), supplemented with 50 units/mL of penicillin/50 μg/mL of streptomycin (Gibco), and under humidified 5% CO2 at 37°C.
The human TM–derived cell line, HTM3, was a gift from Alcon Laboratories.2 (link),27 (link) Trabecular cells were maintained in serum-free DMEM (Gibco) supplemented with 4 mM
Human corneal epithelial progenitor cells were obtained from Zen-Bio, Inc. (Cat# HCEP; Research Triangle Park, NC, USA). These cells were cultured in epithelial culture medium (CnT-Prime; Zen-Bio, Inc.), supplemented with 50 units/mL of penicillin/50 μg/mL of streptomycin (Gibco), and under humidified 5% CO2 at 37°C.
