A 100 mL of rhodamine 6G-carbopol loaded sporopollenin suspension was split in two 200 mL beakers and both suspensions were agitated at the same time at 700 rpm and rhodamine 6G absorbance was measured at various times. Parafilm was placed on each beaker to prevent the dispersions from absorbing carbon dioxide and consequent acidification. At 10 min interval, eight drops of 0.1 M NaOH were added into one of the two beakers. The pH was measured at various times of the experiment. The amount of dye was calculated from the values of the concentration of the dye, which was determined using a UV-vis spectrophotometer (Varian Cary 50 Bio) using the calibration curve of rhodamine in DI water. The values of absorbance were measured at λ = 525 nm, which is the wavelength corresponding to the maximum absorbance of rhodamine in water. The trend after the addition of NaOH was the same for the three attempts, the amount of dye released suddenly rises and then continues increasing less sharply.