Blasts cells were purified by the Ficoll-Hypaque gradient separation method (GE Healthcare). RNA was obtained by using TRIzol (Life Technologies) according to the manufacturer’s recommendations. Isolation of genomic DNA has complied the FlexiGene DNA protocol (Qiagen). ChIP extracts preparation has been carried out following IHEC procedures and as reported in [47 (link), 48 (link), 53 (link), 54 (link)].
Petraglia F., Singh A.A., Carafa V., Nebbioso A., Conte M., Scisciola L., Valente S., Baldi A., Mandoli A., Petrizzi V.B., Ingenito C., De Falco S., Cicatiello V., Apicella I., Janssen-Megens E.M., Kim B., Yi G., Logie C., Heath S., Ruvo M., Wierenga A.T., Flicek P., Yaspo M.L., Della Valle V., Bernard O., Tomassi S., Novellino E., Feoli A., Sbardella G., Gut I., Vellenga E., Stunnenberg H.G., Mai A., Martens J.H, & Altucci L. (2018). Combined HAT/EZH2 modulation leads to cancer-selective cell death. Oncotarget, 9(39), 25630-25646.
Other organizations :
University of Campania "Luigi Vanvitelli", Radboud University Nijmegen, Istituti di Ricovero e Cura a Carattere Scientifico, SDN Istituto di Ricerca Diagnostica e Nucleare, Sapienza University of Rome, Ospedali Riuniti Umberto I, Institute of Genetics and Biophysics, Instituto Biofisika, Centro Nacional de Análisis Genómico, Institute of Biostructure and Bioimaging, University of Groningen, University Medical Center Groningen, European Bioinformatics Institute, Wellcome Trust, Max Planck Institute for Molecular Genetics, Inserm, University of Naples Federico II, University of Salerno, Istituto Pasteur
Manufacturer's recommendations for TRIzol (Life Technologies)
FlexiGene DNA protocol (Qiagen)
IHEC procedures as reported in [47], [48], [53], [54]
controls
Positive controls: Not explicitly mentioned
Negative controls: Not explicitly mentioned
Annotations
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