Quantitative Real-Time PCR Analysis

Quantitative real-time PCR was performed by using LightCycler® 480 Real-Time PCR System (Roche, Germany) and SYBR Green II PCR Master Mix (Takara, Dalian, China). The amplification programme was performed as described in our previous study [40 (link)]. Three biological replicates were carried out for each sample. Transcript levels were calculated using the 2^–∆∆Ct method and normalized using the longan Actin gene (Dlo_028674) [5 (link)] or Arabidopsis Actin2 as an internal control. Genes that were up- or downregulated by at least twofold were considered differentially expressed.

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Jue D., Li Z., Zhang W., Tang J., Xie T., Sang X, & Guo Q. (2024). Identification and functional analysis of the LEAFY gene in longan flower induction. BMC Genomics, 25, 308.

Publication 2024

LightCycler® 480 Real-Time PCR System SYBR Green II PCR Master Mix

Corresponding Organization :

Other organizations : Chongqing University of Arts and Sciences, Southwest University

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Variable analysis

dependent variables

Transcript levels

control variables

Longan Actin gene (Dlo_028674)
Arabidopsis Actin2

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