PEG Hydrogel Formation and Functionalization

PEG-DA gels were formed by adding acrylate-PEG-RGD (2 mM) with various polymer weight percentage solutions of acrylate-PEG-VPM-PEG-acrylate in PBS + 0.05% Irgacure 2959 (Ciba) photoinitiator and exposure to UV light (10 mW cm⁻²) for 10 minutes. Michael-type addition PEG hydrogels (PEG-4A, PEG-4VS, PEG-4MAL) were formed by reacting 4-arm functionalized PEG-macromer with the cell-adhesion peptide GRGDSPC followed by cross-linking with the protease degradable peptide VPM at stoichiometrically balanced 1:1 cysteine to remaining reactive group molar ratio. For most experiments, PEG-4A and PEG-4VS were reacted in PBS + TEA (400 mM, pH 7.4), whereas PEG-4MAL was reacted in PBS + TEA (4 mM, pH 7.4).

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Phelps E.A., Enemchukwu N.O., Fiore V.F., Sy J.C., Murthy N., Sulchek T.A., Barker T.H, & García A.J. (2011). Maleimide cross-linked bioactive PEG hydrogel exhibits improved reaction kinetics and cross-linking for cell encapsulation and in-situ delivery. Advanced materials (Deerfield Beach, Fla.), 24(1), 64-2.

Publication 2011

Acrylate Cell adhesion Cysteine Gels Grgdspc Hydrogels Irgacure 2959 Molar Peg da Peptide Polymer Protease Uv light

Corresponding Organization : Georgia Institute of Technology

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Variable analysis

independent variables

Polymer weight percentage solutions of acrylate-PEG-VPM-PEG-acrylate
Reaction of 4-arm functionalized PEG-macromer (PEG-4A, PEG-4VS, PEG-4MAL) with the cell-adhesion peptide GRGDSPC
Stoichiometrically balanced 1:1 cysteine to remaining reactive group molar ratio

dependent variables

Formation of PEG-DA gels
Formation of Michael-type addition PEG hydrogels

control variables

PEG-DA gels were formed in PBS + 0.05% Irgacure 2959 (Ciba) photoinitiator
PEG-DA gels were exposed to UV light (10 mW cm^-2) for 10 minutes
PEG-4A and PEG-4VS were reacted in PBS + TEA (400 mM, pH 7.4)
PEG-4MAL was reacted in PBS + TEA (4 mM, pH 7.4)
Cross-linking with the protease degradable peptide VPM

controls

Positive control: None specified
Negative control: None specified

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