Serum Biomarker Quantification Protocol

The serum concentrations of TNFR1, TNFR2, and PGRN were measured using enzyme-linked immunosorbent assay (ELISA) kits (cat. # DRT100, DRT200, DPGRN0; R&D Systems, Minneapolis, MN, USA) as previously described [26 (link), 27 (link)]. Serum levels of IL-6 were analyzed using a commercial chemiluminescent enzyme immunoassay (SRL, Tokyo, Japan). The serum CRP was measured through nephelometry, a latex-enhanced immunoturbidimetric assay (QUALIGENT CRP; Sekisui Medical Co., Ltd., Tokyo, Japan) at our institution.

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Gohda T., Murakoshi M., Suzuki Y., Hiki M., Naito T., Takahashi K, & Tabe Y. (2022). Circulating tumor necrosis factor receptors are associated with mortality and disease severity in COVID-19 patients. PLoS ONE, 17(10), e0275745.

Publication 2022

QUALIGENT CRP

Enzyme immunoassay Enzyme linked immunosorbent assay Immunoturbidimetric assay Latex Nephelometry Serum Tnfr1 Tnfr2

Corresponding Organization : Juntendo University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Serum concentrations of TNFR1
Serum concentrations of TNFR2
Serum concentrations of PGRN
Serum levels of IL-6
Serum CRP

control variables

None explicitly mentioned

controls

Positive control: None mentioned
Negative control: None mentioned

Annotations

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