Time-Lapse Imaging of PCa Cells

PCa cells were plated in Hi-Q4 glass-bottom dishes (Ibidi) and cultured in RPMI-1640 containing 10% FBS. Cells were washed with RPMI-1640 containing 0.1% FBS before the addition of Calvarial-CM or recombinant human proteins. Concentrations used for the various recombinant human proteins were empirically derived. Images (20X objective) were acquired every 20 min for 72 h in a BioStation (Nikon) as previously described^{3 (link)}. Data were compiled using NIS-Elements (Nikon) software.

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Yu-Lee L.Y., Lee Y.C., Pan J., Lin S.C., Pan T., Yu G., Hawke D.H., Pan B.F, & Lin S.H. (2019). Bone secreted factors induce cellular quiescence in prostate cancer cells. Scientific Reports, 9, 18635.

Publication 2019

BioStation NIS-Elements

Calvarial Cells Dishes Human Human proteins Recombinant proteins

Corresponding Organization : The University of Texas MD Anderson Cancer Center

Other organizations : Baylor College of Medicine

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Variable analysis

independent variables

Calvarial-CM
Recombinant human proteins

dependent variables

Cell behavior over time (72 h)

control variables

RPMI-1640 containing 10% FBS
RPMI-1640 containing 0.1% FBS

controls

No positive or negative controls were explicitly mentioned in the input protocol.

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